Leica confocal software manual

Confocal Laser Scanning Microscopy (LEICA) This presentation has been put together as a common effort of Urs Ziegler, Anne Greet Bittermann, Mathias Hoechli. Many pages are copied from Internet web pages or from presentations given by Leica, Zeiss and other companies. Please browse the internet to learn interactively all about optics. For. Do you struggle with a variety of instruments, protocols, and software interfaces in your lab? Leica Application Suite X (LAS X) is the one software platform for all Leica microscopes: It integrates confocal, widefield, stereo, super-resolution, and . 10 Living up to Life User Manual Leica TCS SP8 SMD for FCS, FLIM and FLCS.

19 - Leica SP8 Training Guide Understanding Image Quality Pinhole (Confocal Aperture) Ideally, the confocal aperture should be set to the size of the structure(s) you are trying to resolve. However, for example, some sub-cellular structures of interest may be beyond Abbe's diffraction limit and. Quick operation manual for Leica SP8 Confocal/Multiphoton Microscope. Turning ON the system 1-Turn on the Fluorescent light source (number 1) 2- Turn On the NDD power supply. While software is loading do not click any buttons or change the objective - When the software opens, you can choose the objective you. Instructions for using Leica DCM3D confocal microscope. Startup 1. Turn on power to microscope (power unit is located at your feet, next to the computer. Press the front -facing button. Will light up red when on) 2. Check to make sure anti-vibration table is on (power switch in the back of unit) 3.

6) Pinhole- this is the size of the opening in which the laser(s) pass through. For true confocal microscopy, you always want to choose Airy 1. When you hit Airy 1, the red slider will adjust the pinhole to the appropriate diameter based on the objective and laser wavelengths you are using. However, if your signal is weak, you can. 10 Living up to Life User Manual Leica TCS SP8 SMD for FCS, FLIM and FLCS. for confocal and Multiphoton imaging with HYD-RLD plus 2TLD. o Use the third option for confocal and Multiphoton imaging with HYD-RLD - If your project requires, you can turn ON “Resonant scanner” at this point. Otherwise you can leave it “off”. - Press OK and wait for few minutes until software loads up.